Journal: Arteriosclerosis, Thrombosis, and Vascular Biology

Article Title: Platelet Internalization Mediates Ferroptosis in Myocardial Infarction

doi: 10.1161/atvbaha.122.318161

Figure Lengend Snippet: Figure 2. Platelets are internalized by cardiomyocytes. A, Distribution of platelets in myocardial tissue (n=4). Platelets were labeled with CD41 (green), cardiomyocytes were labeled with cTnI (red), and nuclei visualized with DAPI (blue). Scale bar, 500 μm. B, Neonatal mouse cardiomyocytes (CMs) internalize platelets (n=3). CMs were labeled by CM-Dil (red), platelets were labeled by CFSE (green). Scale bar, 100 µm. C, Three-dimensional reconstruction of confocal Z-stack images of CMs co-cultured with platelets to demonstrate uptake and perinuclear localization (n=3; view angle: 30, z-Scaling: 2).

Article Snippet: Primary antibodies included cTnI antibody (1:100, Proteintech, catalog 21652-1- AP) and CD41 antibody (1:200, Santa cruz, sc-365938).

Techniques: Labeling, Cell Culture

Journal: Physiological Reports

Article Title: CXCL10 is a novel anti‐angiogenic factor downstream of p53 in cardiomyocytes

doi: 10.14814/phy2.15304

Figure Lengend Snippet: p53‐induced CXCL10, secreted from cardiomyocytes, inhibited the tube formation of endothelial cells. Culture media were collected from p53‐ or β‐gal‐overexpressing NRCMs. RAOECs were cultured with the conditioned media in the presence of AMG487, an inhibitor of CXCR3. Angiogenesis assay was performed. (a) Representative images were shown. (b) The number of tube formation was measured ( n = 6). * p < 0.05, ** p < 0.01 by one‐way ANOVA followed by Tukey–Kramer test.

Article Snippet: After coating the wells with the extracellular matrix, 5 × 10 5 RAOECs were seeded to each well and cultured with the conditioned media contained AMG 487 (MedChemExpress), a selective antagonist of CXCR3.

Techniques: Cell Culture, Angiogenesis Assay